chicken embryo fibroblasts cef  (Charles River Laboratories)

Journal: bioRxiv

Article Title: The constitutive oncogenic and signaling activities of phosphatidylinositol 3-kinase (PI3K) isoforms p110β and p110δ

doi: 10.1101/2025.06.26.659600

Figure Lengend Snippet: Introduction of the PXXP motif in the ABD of p110α induces a gain of function. (A) Oncogenic activity as measured by transformation of CEF. Representative photographs of cell cultures transfected with 0.5 µg/well of the RCAS(A) vector expressing the respective p110 pro- teins. (B) Integrated band intensities of p-S6 staining of CEFs that express the indicated RCAS constructs. * p < 0.05, ** p <0.01, *** p < 0.005

Article Snippet: Chicken embryo fibroblasts (CEF) were prepared from single, pathogen-free White Leghorn em- bryos (Charles River, N. Franklin, CT) as previously described [ ].

Techniques: Activity Assay, Transformation Assay, Transfection, Plasmid Preparation, Expressing, Staining, Construct

Journal: PLOS Neglected Tropical Diseases

Article Title: Immune response after oral immunization of goats and foxes with an NDV vectored rabies vaccine candidate

doi: 10.1371/journal.pntd.0011639

Figure Lengend Snippet: Embryonated chicken eggs (ECE) (A) , chicken embryo fibroblasts (CEF) (B) , baby hamster kidney cells (BHK-21) (C) , or bovine kidney cells (MDBK) (D) were infected with rNDV or rNDV_G RABV (moi 0.01). Allantoic fluids and cell culture supernatants were harvested at indicated time points after infection (p. i.). Viral titers (TCID 50 /mL) were determined after titration on quail muscle (QM9) cells and subsequent immunostaining. Bar charts depict mean viral titers standard deviation ((A) n = 4, four eggs from one experiment; (B, C, D) n = 4, two samples each from two independent experiments). Asterisks indicate significant differences of mean viral titer (α = 0.05).

Article Snippet: Primary chicken embryo fibroblasts (CEF) were prepared from 10-day-old specific pathogen free (SPF) embryonated chicken eggs (ECE), purchased from Valo, BioMedia (Osterholz-Scharmbeck, Germany) and incubated at 37° with 55% humidity.

Techniques: Infection, Cell Culture, Titration, Immunostaining, Standard Deviation

Journal: PLOS Neglected Tropical Diseases

Article Title: Immune response after oral immunization of goats and foxes with an NDV vectored rabies vaccine candidate

doi: 10.1371/journal.pntd.0011639

Figure Lengend Snippet: Chicken embryo fibroblasts (DF-1) (A) , bovine kidney cells (MDBK) and baby hamster kidney cells (BHK-21) (B) were infected with rNDV or rNDV_G RABV (multiplicity of infection (moi) 5) and harvested 24 h post infection (p. i.). Cell lysates and lysates of purified virions (10 μg per lane) (C) were subjected to SDS-PAGE and subsequently to Western blot analysis. Viral proteins were visualized by immunostaining with respective antibodies. Β-Actin was detected on every cell lysate blot as loading control. Blots of cell lysates are representative of three biological replicates. Blot of purified virions were done once. Uncompartmentalized illustrations can be found in the supporting information .

Article Snippet: Primary chicken embryo fibroblasts (CEF) were prepared from 10-day-old specific pathogen free (SPF) embryonated chicken eggs (ECE), purchased from Valo, BioMedia (Osterholz-Scharmbeck, Germany) and incubated at 37° with 55% humidity.

Techniques: Infection, Purification, SDS Page, Western Blot, Immunostaining, Control